In this study, a MIB episode in a low-latitude drinking water reservoir was investigated. Filamentous cyanobacteria strain Planktothricoides raciborskii was isolated and identified as the main MIB producer in this reservoir. Based on the culturing experiments and in-situ verification, the physiological characteristics of P. raciborskii was explored, and the following conclusions can be drawn: (1) P. raciborskii could grow under a low nutrient condition (TN: 800 μg L$^{-1}$, TP: 10 μg L$^{-1}$), but favored moderate light intensity (54 μmol photon m$^{−2}$⋅s$^{−1}$) and high tempera- ture (30 °C); (2) The long period of high temperature might have inhibited the growth of the bloom-forming Limnothrix and Aphanizomenon, creating the opportunity for the continuous growth of P. raciborskii. (3) The MIB synthesis and extracellular release could be enhanced by high light and temperature, posing a greater risk to water plants in low latitude regions.