重要链接
摘要
Geosmin is often associated with off-flavor problems in drinking water, particularly produced by Anabaena sp. Early detection of geosmin producers and geosmin levels is crucial for managing potential off-flavor events in freshwater systems. This study presents quantitative PCR (qPCR) methods to quantify both the Anabaena sp. population and geosmin-producing potential in freshwater environments. Primer sets targeting the rpoC1 gene of Anabaena sp. and the geosmin synthase gene were designed for this purpose. The qPCR methods were validated using simulated culture blooms and field samples. The density of the rpoC1 gene showed a strong correlation with cell counts, while geosmin synthase gene copies correlated with geosmin concentrations measured by GC-MS. The methods are faster and simpler than traditional techniques, enabling on-site detection and providing timely data for water quality management. They can reduce analysis time from days to just a few hours and are applicable for monitoring in various environmental settings.
引用
@Article{su2013establishment,
title = {Establishment of quantitative PCR methods for the quantification of geosmin-producing potential and *Anabaena* sp. in freshwater systems},
author = {Ming Su and Virginie Gaget and Steven Giglio and Michael Burch and Wei An and Min Yang},
year = 2013,
journal = {Water Research},
volume = 47,
number = 10,
pages = {3444-3454},
url = {https://doi.org/10.1016/j.watres.2013.03.043},
issn = {0043-1354},
doi = {10.1016/j.watres.2013.03.043}
}