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Abstract
Cyanobacteria release 2-methylisoborneol (MIB) as a secondary metabolite. Here, we propose a reverse transcription quantitative real-time PCR (RT-qPCR) based method to evaluate the MIB-producing potential in source water by detecting the MIB-synthesis gene (mic). A MIBQSF/R primer set was designed based on 35 mic gene sequences obtained from 12 pure-cultured MIB-producing strains and 23 sequences from the NCBI database. This primer set successfully identified all known 43 MIB-producing cyanobacterial strains (12 from this study and 31 from the NCBI database), belonging to different genera, showing a wider coverage than previous primer sets. The efficiency of the method was proved by the amplification efficiency (E = 91.23%), R2 of the standard curve (0.999), the limit of detection (LOD, 5.7 fg μL−1), and the limit of quantification (LOQ, 1.86 × 104 gene copies μL−1). Further, the method was verified by the correlation between the mic gene abundance and MIB concentration 50 field samples from different reservoirs (R2 = 0.614, p < 0.001) and one reservoir (R2 = 0.752, p < 0.001), suggesting its potential as an alternative warning tool to evaluate the risk of MIB problems in source water.
Citation
@Article{suruzzaman2022evaluation,
title = {Evaluation of the {MIB}-producing potential based on real-time qPCR in drinking water reservoirs},
author = {Md Suruzzaman and Tengxin Cao and Jinping Lu and Yongjing Wang and Ming Su and Min Yang},
year = 2022,
journal = {Environmental Research},
pages = 112308,
url = {https://www.sciencedirect.com/science/article/pii/S0013935121016091},
doi = {10.1016/j.envres.2021.112308}
}