Early warning of MIB episode based on gene abundance and expression in drinking water reservoirs

曹 腾心; 方 姣; 贾 泽宇; 朱 宜平; 苏 命; 张 琪; 宋 一超; 于 建伟; 杨 敏

doi:10.1016/j.watres.2023.119667

摘要

细胞内2-甲基异莰醇（MIB）产量因蓝藻在不同条件下的变化而异，但其因果机制尚不明确，这导致了MIB预测的低效性。通过对青草沙（QCS）水库中假鱼腥藻（Pseudanabaena cinerea）引发的一次MIB事件的深入实地调查，我们证明了MIB合成相关的mic基因丰度（DNA）和表达量（RNA）可能可作为MIB生成的预警参数。研究发现，mic基因的DNA和RNA丰度分别在MIB浓度峰值之前10天和7天达到高峰。此外，RNA丰度（R² = 0.45, p < 0.01）与MIB的相关性略高于DNA丰度（R² = 0.37, p < 0.01），表明假鱼腥藻的生长条件可能与mic基因的表达条件略有不同。这一推论通过培养实验得到验证。在实验中，细胞的最高生长速率出现在36 μmol photons m^-2 s^-1的光照强度下，而细胞内MIB产量和mic基因表达水平的峰值则出现在85 μmol photons m^-2 s^-1的光照强度下。我们的研究明确表明，光强是控制培养实验中mic基因表达以及水库实际MIB事件的关键调控因素。此外，我们开发了一个以mic基因丰度为指标的MIB事件预警模型，并在另外两个水库中验证了其有效性。我们的研究成果突出了光强对mic基因表达和MIB合成的影响，并提供了一种用于预测MIB事件的预警工具。这对于水源管理机构而言具有重要意义。

Introduction by AI

摘要

图摘要

内容概览

The spatial and temporal distributions of MIB concentrations during the MIB episode of QCS Reservoir in 2021.

Cyanobacterial community (obtained by microscopic cell counting) in QCS Reservoir at the phylum level

Cyanobacterial community (obtained by microscopic cell counting) in QCS Reservoir at the genus level (top 20% genera, A), the mic gene order (B) and proportion of relative abundances of MIB-producing cyanobacteria determined by mic gene sequencing (C).

引用与导出

@Article{cao2023early,
    title       = {Early warning of MIB episode based on gene abundance and expression in drinking water reservoirs},
    author      = {Tengxin Cao and Jiao Fang and Zeyu Jia and Yiping Zhu and Ming Su and Qi Zhang and Yichao Song and Jianwei Yu and Min Yang},
    year        = 2023,
    journal     = {Water Research},
    volume      = 231,
    pages       = 119667,
    url         = {https://www.sciencedirect.com/science/article/pii/S0043135423001021},
    issn        = {0043-1354},
    doi         = {10.1016/j.watres.2023.119667}
}