Evaluation of the MIB-producing potential based on real-time qPCR in drinking water reservoirs


摘要

Cyanobacteria release 2-methylisoborneol (MIB) as a secondary metabolite. Here, we propose a reverse tran- scription quantitative real-time PCR (RT-qPCR) based method to evaluate the MIB-producing potential in source water by detecting the MIB-synthesis gene (mic). A MIBQSF/R primer set was designed based on 35 mic gene sequences obtained from 12 pure-cultured MIB-producing strains and 23 sequences from the NCBI database. This primer set successfully identified all known 43 MIB-producing cyanobacterial strains (12 from this study and 31 from the NCBI database), belonging to different genera, showing a wider coverage than previous primer sets. The efficiency of the method was proved by the amplification efficiency (E = 91.23%), $R^2$ of the standard curve (0.999), the limit of detection (LOD, 5.7 fg μL$^{−1}$), and the limit of quantification (LOQ, $1.86\times 10^4$ gene copies μL$^{−1}$). Further, the method was verified by the correlation between the mic gene abundance and MIB concen- tration 50 field samples from different reservoirs ($R^2 = 0.614$, p < 0.001) and one reservoir ($R^2 = 0.752$, p < 0.001), suggesting its potential as an alternative warning tool to evaluate the risk of MIB problems in source water.

出版物
In Environmental Research
曹腾心
曹腾心
博士
陆金平
陆金平
博士研究生
苏命
苏命
副研究员

我的研究方向:水源地水质安全保障,主要针对水源地由于藻类爆发引起的水体嗅味问题开展研究。

杨敏
杨敏
研究员, 中国科学院生态环境研究中心副主任